It was the third time they had set their club record in the window.
Flush from the proceeds of a new £5.14bn domestic television rights deal, Premier League clubs broke through the one billion pounds barrier in one transfer window for the first time. They have to keep up.
Analysts predict European spending will easily be the highest yet when the two-month transfer window closes, between 1600 GMT for the Bundesliga and 2200 GMT for the Premier League.
That's also far lower than what was spent on the summer transfer window's biggest reunion. The fee is rumoured to be around £34 million, according to the BBC.
He signed a five-year contract and is thought to have cost Spurs around £30mn (B1.36bn). "It's time for me to win some titles", Real Madrid academy graduate Alonso said.
Sissoko, 27, looked poised to join Everton for much of the day, only for Tottenham to reportedly pip the Merseyside club with a late bid.
Hart and Nasri are former City stalwarts, while Ivory Coast striker Bony arrived from Swansea City a year ago at a cost of £25 million. Luiz's return was preceded by Georges-Kevin Nkoudou's move from Marseille to Tottenham and Sunderland signing Didier Ndong from Lorient.
Everton consoled themselves with a loan deal for West Ham United's Ecuadorean forward Enner Valencia.
Balotelli, Liverpool's wayward Italian striker, was on the verge of joining French club Nice on a season-long loan, having spent last season on loan at former club AC Milan.
A host of temporary moves in La Liga saw the return of Diego Lopez from Milan to Espanyol, and Manchester City duo Samir Nasri and Eliaquim Mangala joined Sevilla and Valencia.
Chelsea had already landed Fiorentina's former Bolton left-back Marcos Alonso for a reported £23 million earlier in the day.
Leicester were among 13 Premier League clubs to break their club transfer record, paying an initial 30 million euros ($44.43 million) to sign Sporting Lisbon striker Slimani. Chelsea announced on their official website that a fee had been agreed upon between the two clubs.
Burnley were another club to make the biggest purchase in their history, buying Irish midfielder Jeff Hendrick from Derby County for a reported pound sterling10 million.
Liverpool has offloaded Mario Balotelli to French club Nice, two years after signing the troubled striker.
English Premier League clubs swelled the bank accounts of continental rivals in a summer of record-breaking spending that ended Wednesday with the biggest shock of the transferwindow: David Luiz's return to Chelsea.
HULL CITY last night signed Manchester United midfielder James Weir.
In Germany, Werder Bremen signed German winger Serge Gnabry from Arsenal and Hamburg made a 10 mn euros (B386mn) move to bring Brazilian defender Douglas to the club from Atletico Mineiro.