England limited-overs skipper Eoin Morgan came in support of the decision to rest Durham all-rounder Ben Stokes for the one-off Twenty20 worldwide against West Indies to be played on Saturday in Chester-le-Street.
This time, paceman Brathwaite damaged England with the ball when, in his only match of the tour - - he was not selected for the Test or one-day global legs - he took three for 20 runs in 3.3 overs.
They were separated, however, following a calamitous piece of running between the wickets - Gayle was first guilty of ball watching after chopping to point, then, after some hesitation, ambled for a single, refusing to be hurried by Jason Roy's throw to Liam Plunkett or dive to save himself.
Walton recovered, however, and he was behind the stumps as West Indies completed a 21-run win - their 11th in 15 Twenty20 internationals against England, a sequence including last year's World Twenty20 final triumph in India.
After a highly contested Test series between England and West Indies that went in favour of the hosts we get ready to embrace the limited-overs format.
Hales's exit was the start of a slump that saw England lose three wickets for four runs as 64 for one soon became 68 for four.
Brathwaite ignited the collapse when he had Hales clean bowled off a straight delivery, then Joe Root (17) caught in the next over off Ashley Nurse.
A heavy shower blew into the Emirates Riverside with just seven balls of the Windies' innings remaining and Eoin Morgan's team in the ascendancy - although the radar suggested further interruptions would be minimal.
And the 31-year-old insists the six upcoming matches against West Indies will provide a stern challenge.
"It's the batting certainly", Morgan said.
Chris Gayle (40) and Evin Lewis (51) got West Indies off to a flying start as they raced to 72 without loss in just six overs after England captain Eoin Morgan won the toss and fielded.
But July saw him return to West Indies duty in a Twenty20 worldwide against India in Kingston.
Following the one-off T20I, both England and West Indies will face other in a five match ODI series to be played between 19th and 29th September.
"Evin Lewis has has been striking it cleanly, there were even times when Chris Gayle had to play second fiddle", said Brathwaite.