Officials said officers were patrolling the neighborhood when a 911 call came in of shots fired around 4:51 a.m.
Spokesman Steve Hegarty said detectives can't immediately say whether the shooting is related to last month's killings where three people were slain during a 10-day span.
The three victims didn't know one another, but each person rode the bus and were alone when they were shot on the street.
There is a massive police presence in the area searching for the suspect. Witnesses described seeing a black man in his early 20's, wearing a dark hooded jacket, running from the area where this shooting took place.
The Tampa Police Department is investigating a suspicious death Tuesday morning in the Seminole Heights neighborhood, where fears of a possible serial killer gripped the community last month.
This comes almost a month after a killer shot and killed three people over the span of 10 days in Seminole Heights. When officers arrived, they found the body of Ronald Felton, 60, in the street.
On Oct. 13, victim Monica Hoffa, 32, was found in a vacant lot in the 1000 block of New Orleans Avenue in East Tampa.
The first victim, 22-year-old Benjamin Mitchell, was shot and killed on October 9 while he was waiting at a bus stop. And on October 19, Anthony Naiboa, 20, was shot after taking the wrong bus home from his new job.
On Oct. 9, victim Benjamin Mitchell, 22, was waiting for a bus on North 15th Street and Frierson Avenue in Tampa. They've asked residents within their perimeter to remain indoors until further notice.
"We will hunt this person down until we find them", Tampa Mayor Bob Buckhorn said during a press conference Tuesday morning. Tampa Police Chief Brian Dugan believes that the suspect is a resident of the Seminole Heights neighborhood.