Uruguay were the only side to come through the group stage in Russian Federation without letting in a goal.
Defensive midfielder William Carvalho is a doubt after he missed a training session on Thursday.
Suarez was frustrated during the opening game against Egypt but appears to be coming to a boil just at the right time - he has managed to avoid any further World Cup controversies so far as well, following 2010's handball and 2014's biting.
The result, combined with Spain's 2-2 draw with Morocco and an earlier win for Uruguay over hosts Russian Federation in Group A, means Portugal will play Uruguay in Sochi on June 30, while Group B winners Spain will face Russian Federation in Moscow on July 1.
Uruguay coach Oscar Tabarez: "We need to improve very quickly [from the performance against Russia], because any match in the round of 16 will be extremely hard". Nearly irrespective of who those wins came against, Uruguay will be on a high as one of only three teams to triumph in all their group games in Russia-Croatia and Belgium are the other two. Skipper Diego Godin will be in charge of shackling Ronaldo on Saturday evening.
Uruguay were the first team to win all three group games without conceding a goal since Argentina in 1998.
Who wins Portugal-Uruguay? And where does all the value lie?
The question is whether Ronaldo has it in his locker to tear down Uruguayan walls regardless and add to his tally of four goals, with Portugal's supporting cast hoping their record scorer can dazzle once again in Russian Federation.
- Luis Suarez has scored seven World Cup goals for Uruguay, second only to Oscar Miguez (eight) - Suarez scored a brace against South Korea in his only previous appearance in the round of 16 back in 2010.
The unquestionable driving force and inspiration for Fernando Santos' men, Ronaldo flew out of the blocks in this tournament but missed a penalty and might have been sent off in a tempestuous encounter with Iran.
The five-times world player of the year may have filled his boots against Godin on several occasions, including two hat-tricks against Atletico, but the defender has also kept the prolific Portuguese quiet in many games, preventing him from finding the net in 13 of their last 20 meetings.
"We have our weapons and we are trying to win", he said, according to ESPN.
This is Fernando Santos' fourth major tournament in a row as manager.