The five-time Ballon d'Or victor was in obvious pain, although Barca's medical team dressed his right arm and it initially appeared as though he may attempt to play on.
Messi was unable to play on though and Barcelona confirmed shortly after full-time that tests have revealed a "fracture of the radial bone in his right arm". "He will be out for approximately three weeks", stated F.C. Barcelona on Twitter.
"We will notice his absence", Barcelona coach Ernesto Valverde said.
"Messi's injury does not have to affect us mentally", Gerard Pique said.
Maradona made headlines earlier this month when he criticised Messi by saying "it's useless trying to make a leader out of a man who goes to the toilet 20 times before a game".
Messi he was hurt midway through the first-half in an innocuous-looking challenge with Franco Vazquez.
Sevilla host Turkish side Akhisar in the Europa League on Thursday before hosting Huesca next Sunday in La Liga.
Lionel Messi is set to miss some crucial games for Barcelona over the coming weeks.
We all know what the opponent thinks when they play Leo.
"With this initiative, we continue to fight against the cruel disease that is cancer.Collaborating makes this easier".
After Messi's exit, Luis Suarez added to Barcelona's lead from the penalty spot before a frantic finish which saw Pablo Sarabia pull a goal back for Sevilla, Ivan Rakitic volley in a fourth Barca goal and Luis Muriel score a stoppage-time consolation for the visitors.
Barca then place Leonesa in the Copa del Rey, having a trip to Rayo Vallecano, a visit to Inter and a home tie with Real Betis before the mid-November worldwide break.