After apparent footage of the Arsenal players inhaling the laughing gas emerged recently, the London club released a brief statement addressing the situation.
A number of Gunners stars were in attendance at the "champagne and vodka party" in a VIP area of a West End Nightclub in August prior to the Premier League season getting underway.
An Arsenal spokesperson told the BBC players would be "spoken to and reminded of their responsibilities".
Other players such as Raheem Sterling and Kyle Walker have been seen using the gas in the past, with the City fullback apologising for using the substance back in 2013.
Nitrous oxide, known as "hippy crack" can cause dizziness, uncontrollable laughter and hallucinations if inhaled....
The North London club sit in fifth in the Premier League table, only behind fourth-placed Chelsea on goal difference.
Arsenal endured a challenging start to the season, and manager Unai Emery found himself under a huge amount of pressure.