"This week, the company began sending out emails to users informing them that its user guidelines have been updated to "[make] it clear that all types of ad blockers, bots, and fraudulent streaming activities are not permitted".
Users who will keep their ad-blocking software active after this date may get their accounts suspended or banned entirely.
That figure represented 1% of total users, and 2% of people using the ad-supported service.
Spotify has updated its Terms of Service agreement, implementing an explicit ban on the use of ad blockers. Some users have found ways around these ads, though, such as modified applications and ad-blocking tech.
The Terms and Conditions of Use state that "circumventing or blocking advertisements in the Spotify Service, or creating or distributing tools designed to block advertisements in the Spotify Service" will result in the removal or suspension of your account.
But thanks to the new terms of service, Spotify has the freedom to immediately shutdown an account without warning. Spotify said past year that it estimated two million people - one per cent its total users, and 2 per cent of people using the ad-supported service - were using its free service in a way that generated no revenue for the company or musicians.
An eMarketer report previous year said that a quarter of USA internet users, or about 70 million people, deploy ad blockers. "The format is really evolving and while podcasting is still a relatively small business today, I see incredible growth potential for the space and for Spotify in particular".