Japanese-bred Kluger, a G2 victor in Japan for Carrot Farm who had not won a race since 2016, finished second, beaten 1 1/2 lengths, with Godolphin's veteran G1-winning Hartnell a distant third in the field of nine older runners going 2,000 meters (1 1/4 miles) on good turf in front of a packed house of 43,833 at Randwick racecourse.
The seven-year-old, who survived a scare on Thursday when kicking out at railings and leaving a hole in an advertising board after her final gallop, came with her customary run from half a mile out, as the grandstands began to erupt.
Bowman brought Winx out slowly from the traps early on before settling into sixth place, and then made a signature dash to the finish on the 600-metre mark.
An emotional Bowman said the number of people who had come to see Winx's last-ever race was overwhelming. "She's just an incredible horse for everybody to be part of and I'm just a small cog in the wheel". "Everyone watching around the world", he said.
A 20th Randwick score will take Winx's career earnings to A$26.3 million, an amount well north of US$19 million.
"America, Europe. Africa. All over Australia".
The world's top-rated turf horse sprinted home in front of 50,000 people at Royal Randwick in Sydney.
"There is really nothing more for me to say".
The win was also the 100th Group 1 victory for trainer Chris Waller.
"It sure has. I hadn't thought about winning a race like today ever in my lifetime".
He said it was a special moment to share the win with Winx's fans around the country. I've had to run against her to get to the races I wanted to.
"Whether they are here at Randwick today, but all around Australia, New Zealand, the world". Her 37 career victories match the record of another Australian racing legend, Phar Lap, who raced from 1929-'32. "But thanks for appreciating her for what she is".