Their indictments came after AG Herring's Animal Law Unit dug into the pair's relationship and determined each man was trafficking lion cubs.
Wilson has been charged with one felony count of wildlife trafficking, one felony count of conspiracy to wildlife traffic and 17 misdemeanor charges.
A second daughter, Tawny Antle, faces one misdemeanour count of cruelty to animals and one misdemeanour count of violating the Endangered Species Act.
Antle could not immediately be reached for comment.
You may remember Doc Antle as the owner of Myrtle Beach Safari in SC.
His two daughters were also named in the indictment.
In a statement obtained by the New York Times, Antle said: "I categorically deny any act or conduct that could ever be considered as 'animal cruelty.' I have spent my entire professional life promoting the welfare and conservation of big cats and other species".
Herring's office alleged that Antle and "roadside zoo" owner Keith Wilson conspired to commit lion cub trafficking "between Virginia and SC".
Detectives discovered the two trafficked lion cubs between Virginia and SC which prompted a search of Antle's property in December of 2019. Investigators accused both Antle and Wilson, owner of Wilson's Wild Animal Park in Virginia, of trafficking the cubs between Virginia and SC.
Wilson and his nephew Christian Dall'Acqua, who were not featured in "Tiger King", were previously indicted on 46 counts of animal cruelty by the grand jury in Frederick County in November 2019, the attorney general said. Authorities say they seized 119 animals, including lions, tigers, bears, camels, goats, water buffalo, and more from Wilson's roadside zoo and claim he got some of the animals from Antle. A trial date has been set in the Wilson and Dall'Acqua case for June 21-25, 2021.
The safari park owner, who appeared in the Netflix documentary earlier this year, is accused of allegedly trafficking lion cubs between Virginia and SC, along with Keith A Wilson, another animal park owner.
His role in the series was overshadowed by the botched murder-for-hire plot Joe Exotic, previous owner of The Greater Wynnewood Exotic Animal Park, launched against big cat advocate, Carole Baskin.
Last month, Joe Exotic asked for an official pardon from President Donald Trump.
On Wednesday, the docuseries' Tim Stark, the founder of the self-proclaimed animal refuge Wildlife in Need in IN, was arrested in Granville, N.Y., according to local news outlets.
Even before he became somewhat of a household name following the release of Tiger King Antle's zoo had been a popular tourist attraction and Antle even worked with Britney Spears iconic 2001 performance at the VMA, which included an on-stage tiger.